ANTIMICROBIAL SUSCEPTIBILITY AND MOLECULAR CHARACTERIZATION OF STAPHYLOCOCCUS AUREUS FROM MASTITIC COWS
- Project Research
- 1-5 Chapters
- Abstract : Available
- Table of Content: Available
- Reference Style: APA
- Recommended for : Student Researchers
- NGN 3000
ABSTRACT
This study investigated the antimicrobial susceptibility and molecular characterization of Staphylococcus aureus in mastitic cows in southern part of Kano State of Nigeria. A total of two hundred and thirty four (234) milk and swab samples were collected from six (6) local government areas of Kano State in northern part of Nigeria. Staphylococcus aureus was isolated from samples and identified using standard microbiological procedures. The antimicrobial susceptibility of the isolates was determined using Kirby- Bauer disc diffusion technique as described by the Clinical and Laboratory standards institute (CLSI). Resistant isolates were tested for Beta-lactamase production. A total of 200 (85.5%) have staphylococcal isolates, of which 141 (70.5%) were Staphylococcus aureus (coagulase positive staphylococci, CPS), 59 (29.5%) were coagulase negative staphylococci (CoNS) and 6 (2.9%) were non- Staphylococcal (rods) isolates. The antibiotic susceptibilities of the Staphylococcus aureus isolates were in the following order: ofloxacin (99.3%) > ciprofloxacin (98.6%) > gentamicin (91.5%) > cephalexine (56%) > sulphamethoxazole/trimethoprim (46.1%) > tetracycline (43.3%) > cefuroxime (33.3%) > cefoxitin (2.1%) > amoxicillin (1.4%) > ampicillin (1.4%). Resistance to three or more antimicrobials was presented in 98.6% of the Staphylococcus aureus isolates. Of the 141 Staphylococcus aureus isolates tested for β-lactamase production, 31 (22.5%) were β lactamase producers. However, of the 31 β-lactamase producers, only 13 (41.9%) were multidrug resistant to cefoxitin, Ampicillin and cephalexine. PCR assay was used to detect mecA and blaZ genes in multi-drug resistant Staphylococcus aureus isolates that were β-lactamase producers. The MDRStaphylococcus aureus was also tested by latex agglutination for presence of PBP2a. PCR results indicated none of the isolates showed any amplification to both mecA gene and blaZ gene. All the Beta-lactamase producing MDR- Staphylococcus aureus isolates tested showed negative reaction to latex agglutination test which was also an indication of absence of Pinicillin binding protein (PBP2a). In conclusion, high isolation of Staphylococcus aureus (70.5%) causing bovine mastitis was observed in this study in which both mecA and blaZ genes were absent
Related Project Materials
DESIGN AND IMPLEMENTATION OF A WEB-BASED DRUG INVENTORY MANAGEMENT SYSTEM
ABSTRACT
Inventory management is one of the necessities of every business institution. Every organization possesses its...
A STUDY OF THE CAUSES AND EFFECTS OF STRESS ON SECRETARY'S JOB PERFORMANCE IN BUSINESS ORGANIZATIONS
ABSTRACT
The purpose of this work was to find out the effects of stress on the secretary’s job performance. A ques...
NIGERIA: POSITIONING RURAL ECONOMY FOR IMPLEMENTATION OF SUSTAINABLE DEVELOPMENT GOALS
Abstract
Nigeria as nation has over the years engaged in lots of developmental activities without actions which makes a...
AN ASSESSMENT OF THE FUNCTIONS OF SOCIAL MEDIA IN CREATING AWARENESS ABOUT DOMESTIC VIOLENCE IN NIGERIA
Background to the Study
There has been disturbing news of domestic violence in every nook and cranny of...
THE EFFECT OF NIGERIAN PIDGIN ENGLISH ON THE ACADEMIC PERFORMANCE OF UNIVERSITY STUDENT
ABSTRACT
This study is on the effect of Nigeria pidgin English on the academic performance of university student....
MASS MEDIA AND ITS ROLE IN INFLUENCING THE RATE OF CRIME AMONG YOUTHS IN THE SOCIETY
ABSTRACT
This study was carried out to examine mass media and its role in influencing the rate of...
EFFECT OF MOTIVATION ON EMPLOYEES IN THE SERVICE INDUSTRY: A CASE STUDY OF BENIN ELECTRICITY DISTRIBUTION COMPANY (BEDC)
ABSTRACT
If the managers are able to understand, predict and control employee behavior, then they should have the knowle...
A DESIGN AND IMPLEMENTATION OF AN ONLINE TRADING COMMUNITY WEBSITE
INTRODUCTION
The Internet has become a widely recognised method of technology for doin...
DESIGN AND IMPLEMENTATION OF AN ONLINE DRIVING SCHOOL MANAGEMENT SYSTEM
Abstract
With the rapid development in technology, there is a need to develop a management system for drive-training enterprise. Driving-...
PRIOR KNOWLEDGE AND PERCEPTIONS OF DRUG ADDICTS ON PSYCHOACTIVE SUBSTANCE ABUSE INFORMATION COMMUNICATED BY NDLEA IN NIGERIA
Background to the Study
Psychoactive substance abuse is a major problem in the world that causes mental disorder, social...
